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KMID : 0545119990090030318
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 3 p.318 ~ p.322
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Expression of Human Thrombopoietin in Insect Cells by Polyhedrin-gp64 Dual Promoter-Based Baculovirus Vector System
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Koh, Yeo Wook
Park, Sang Kyu/Kim, Kun Soo/Kim, Seong Ryong/Lee, Jeong Kug/Yang, Jai Myung
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Abstract
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A new baculovirus transfer vector (pPGP404) was constructed to increase the expression level of human thrombopoietin (hTPO) in insect cells. In pPGP404, hTPO was cloned next to the AcNPV polyhedrin-gp64 dual promoter and the leader sequence of hTPO was substituted with that of gp64. A recombinant baculovirus, AcPGP404, was constructed by using pPGP404 as a transfer vector. hTPO was expressed in AcPGP404-infected TN5 cells and it was observed that the expression levels of hTPO in TN5 cells increased three-fold (6.0 ㎍/ml^-1) compared to the level expressed under the control of the polyhedrin single promoter. These results indicate that the polyhedrin-gp64 dual promoter system would be useful for expression in large quantities of recombinant proteins in insect cells.
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KEYWORD
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